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parental control hek bluetm null cell line  (InvivoGen)


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    InvivoGen parental control hek bluetm null cell line
    Parental Control Hek Bluetm Null Cell Line, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 581 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/parental control hek bluetm null cell line/product/InvivoGen
    Average 96 stars, based on 581 article reviews
    parental control hek bluetm null cell line - by Bioz Stars, 2026-03
    96/100 stars

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    B. theta VPI-5482 conditioned media stimulates human and murine TLR2. ( A ) Mouse TLR2 reporter HEK293 cells or the parental cell line (null2) were stimulated with B. theta VPI-5482 conditioned media or TYG control media at the indicated doses for 20 h and TLR2 stimulation was assessed by quantification of secretion of embryonic alkaline phosphatase (SEAP)-mediated hydrolysis of detection media which can be measured by absorbance at 620 nm. Background signal was corrected by subtracting null2 absorbance values from mTLR2 absorbance values for each individual treatment group. ( B–D ) Human TLR2 ( B ), TLR2/6 ( C ), and TLR2/1 ( D ) reporter HEK293 cells, or the parental cell line, <t>null1</t> for hTLR2, and TLR2KO-TLR1/6 for hTLR2/6 and hTLR2/1 cells, were stimulated with B. theta VPI-5482 conditioned media or TYG control media at the indicated doses for 20 h and TLR2 stimulation was assessed by quantification of secretion of embryonic alkaline phosphatase (SEAP)-mediated hydrolysis of detection media which can be measured by absorbance at 620 nm. Background signal was corrected for by subtracting null1 absorbance values from hTLR2 absorbance values, or hTLR2KO-TLR1/6 absorbance values from either hTLR2/1 or hTLR2/6 absorbance values for each individual treatment group. Data points represent the mean and the error bars represent the standard deviation, n = 2 technical replicates per dose ( A–D ). Graph is representative of three experiments ( A–D ). Statistical significance was determined using a Student’s t test on the area under the curve ( A–D ). **** P ≤ 0.0001.
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    B. theta VPI-5482 conditioned media stimulates human and murine TLR2. ( A ) Mouse TLR2 reporter HEK293 cells or the parental cell line (null2) were stimulated with B. theta VPI-5482 conditioned media or TYG control media at the indicated doses for 20 h and TLR2 stimulation was assessed by quantification of secretion of embryonic alkaline phosphatase (SEAP)-mediated hydrolysis of detection media which can be measured by absorbance at 620 nm. Background signal was corrected by subtracting null2 absorbance values from mTLR2 absorbance values for each individual treatment group. ( B–D ) Human TLR2 ( B ), TLR2/6 ( C ), and TLR2/1 ( D ) reporter HEK293 cells, or the parental cell line, null1 for hTLR2, and TLR2KO-TLR1/6 for hTLR2/6 and hTLR2/1 cells, were stimulated with B. theta VPI-5482 conditioned media or TYG control media at the indicated doses for 20 h and TLR2 stimulation was assessed by quantification of secretion of embryonic alkaline phosphatase (SEAP)-mediated hydrolysis of detection media which can be measured by absorbance at 620 nm. Background signal was corrected for by subtracting null1 absorbance values from hTLR2 absorbance values, or hTLR2KO-TLR1/6 absorbance values from either hTLR2/1 or hTLR2/6 absorbance values for each individual treatment group. Data points represent the mean and the error bars represent the standard deviation, n = 2 technical replicates per dose ( A–D ). Graph is representative of three experiments ( A–D ). Statistical significance was determined using a Student’s t test on the area under the curve ( A–D ). **** P ≤ 0.0001.

    Journal: Microbiology Spectrum

    Article Title: BT1549 coordinates the in vitro IL-10 inducing activity of Bacteroides thetaiotaomicron

    doi: 10.1128/spectrum.01669-24

    Figure Lengend Snippet: B. theta VPI-5482 conditioned media stimulates human and murine TLR2. ( A ) Mouse TLR2 reporter HEK293 cells or the parental cell line (null2) were stimulated with B. theta VPI-5482 conditioned media or TYG control media at the indicated doses for 20 h and TLR2 stimulation was assessed by quantification of secretion of embryonic alkaline phosphatase (SEAP)-mediated hydrolysis of detection media which can be measured by absorbance at 620 nm. Background signal was corrected by subtracting null2 absorbance values from mTLR2 absorbance values for each individual treatment group. ( B–D ) Human TLR2 ( B ), TLR2/6 ( C ), and TLR2/1 ( D ) reporter HEK293 cells, or the parental cell line, null1 for hTLR2, and TLR2KO-TLR1/6 for hTLR2/6 and hTLR2/1 cells, were stimulated with B. theta VPI-5482 conditioned media or TYG control media at the indicated doses for 20 h and TLR2 stimulation was assessed by quantification of secretion of embryonic alkaline phosphatase (SEAP)-mediated hydrolysis of detection media which can be measured by absorbance at 620 nm. Background signal was corrected for by subtracting null1 absorbance values from hTLR2 absorbance values, or hTLR2KO-TLR1/6 absorbance values from either hTLR2/1 or hTLR2/6 absorbance values for each individual treatment group. Data points represent the mean and the error bars represent the standard deviation, n = 2 technical replicates per dose ( A–D ). Graph is representative of three experiments ( A–D ). Statistical significance was determined using a Student’s t test on the area under the curve ( A–D ). **** P ≤ 0.0001.

    Article Snippet: Parental cell lines null1 (Invivogen, Cat #hkb-null1), null2 (Invivogen, Cat #hkb-null2), and the control cell line for hTLR2/1 and hTLR2/6 reporter lines, human TLR2KO-TLR1/6 (Invivogen, Cat #hkb-htlr2k16; cells are deficient in TLR1 and TLR6) were also used.

    Techniques: Control, Standard Deviation